| 货号 | 2073T |
| 同种亚型 | Rabbit |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Naked2 (C67C4) Rabbit mAb detects endogenous level of Naked2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6). |
| 存放说明 | -20C |
| 计算分子量 | 59, 61 |
| 参考文献 | 1 . Zeng, W. et al. (2000) Nature 403, 789-795. 2 . Wharton, K.A. et al. (2001) Dev Biol 234, 93-106. 3 . Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026. 4 . Yan, D. et al. (2001) Proc Natl Acad Sci U S A 98, 3802-7. 5 . Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978. 6 . Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576. |
Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb. 使用Naked2 (C67C4) 兔单抗对多种细胞提取物进行western blot分析。 |
