| 货号 | 2087T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P |
| 目标/特异性 | Axin1 (C76H11) Rabbit mAb detects endogenous levels of total Axin1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).Axin1（轴心抑制蛋白1）和Axin2为多结构域支架蛋白，并负向调控Wnt信号通路。Axin1与APC, GSK-3β, Dvl和 β-catenin相互作用促进GSK-3β调控的磷酸化并随后β-catenin的降解（1,2）。由于Wnt引起的细胞刺激，Axin1被磷酸化的LRP 5/6招募到细胞膜上,这个过程被认为是Wnt信号通路激活的关键（3,4）。除了在Wnt信号上的作用外，Axin1和MEKK1形成复合物，并激活c-jun氨基末端激酶（JNK/SAPK）（5）。Axin2(也称Conductin 或Axil)在小鼠体内功能上能代替Axin1（6）。Axin2自身直接是Wnt信号通路的靶标，并因此通过负向反馈机制参与控制Wnt通路的强度和持续时间（7-9）。 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Luo, W. and Lin, S.C. Neurosignals 13, 99-113. 2 . Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236. 3 . Zeng, X. et al. (2005) Nature 438, 873-877. 4 . Davidson, G. et al. (2005) Nature 438, 867-872. 5 . Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254. 6 . Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376. 7 . Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183. 8 . Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193. 9 . Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665. |
Western blot analysis of extracts from various cell lines using Axin1 (C76H11) Rabbit mAb. 使用Axin1 (C76H11) 兔单抗对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Axin1 (C76H11) Rabbit mAb. 使用Axin1 (C76H11) Rabbit mAb兔单抗对石蜡包埋的人结肠癌组织进行免疫组化分析。 |
