| 货号 | 2114T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Rictor (53A2) Rabbit mAb detects endogenous levels of total Rictor protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
| 参考文献 | 1 . Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302. 2 . Sarbassov, D.D. et al. (2005) Science 307, 1098-101. 3 . Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416. 4 . Ali SM and Sabatini DM (2005) J Biol Chem 280, 19445–8 |
Western blot analysis of extracts from various cell lines, using Rictor (53A2) Rabbit mAb. Western blot方法检测分析各种细胞株的提取物，使用的抗体是Rictor (53A2) Rabbit mAb兔单抗。 |
