| 货号 | 5521T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Monkey,Bovine,Pig, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | 14-3-3 η (D23B7) Rabbit mAb recognizes endogenous levels of total 14-3-3 η. This antibody shows weak cross-reactivity with 14-3-3 γ but does not detect any other 14-3-3 family isoforms. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes which modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | 1 . Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9. 2 . Mackintosh, C. (2004) Biochem J 381, 329-42. 3 . Dougherty, M.K. and Morrison, D.K. (2004) J Cell Sci 117, 1875-84. 4 . Yaffe, M.B. (2002) FEBS Lett 513, 53-7. 5 . Bridges, D. and Moorhead, G.B. (2004) Sci STKE 2004, re10. |
Isoform specificity of 14-3-3 η (D23B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each) were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 η (D23B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).14-3-3 η (D23B7) Rabbit mAb亚型的特异性。使用14-3-3 η (D23B7) Rabbit mAb (上图)和GST (91G1) Rabbit mAb #2625 (下图)，通过SDS-PAGE电泳、转移至硝酸纤维素膜，随后免疫印迹（Western Blot）分析重组、纯化和GST标记的14-3-3 蛋白亚型(2 µg each)水平。 | |
Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb.使用14-3-3 η (D23B7) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞系中全细胞裂解物。 |
