| 货号 | 2117T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. The antibody does not recognize the related proteins RhoB or RhoC. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
| 参考文献 | 1 . DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol. 15, 356-363. 2 . Wennerberg, K. and Der, C.J. (2004) J. Cell Sci. 117, 1301-1312. 3 . Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9. 4 . Bi, D. et al. (2005) Circ. Res. 96, 890-897. 5 . Kimura, K. et al. (2000) J. Biol. Chem. 275, 17233-17236. 6 . Barry, S.T. and Critchley, D.R. (1994) J. Cell Sci. 107 ( Pt 7), 2033-2045. 7 . Van Keymeulen, A. et al. (2006) J. Cell Biol. 174, 437-445. |
Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb. 使用RhoA (67B9) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中RhoA蛋白水平。 | |
Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoA (67B9) Rabbit mAb. 使用RhoA (67B9) Rabbit mAb，免疫印迹（Western Blot）分析重组的GST标签的RhoA、RhoB和RhoC蛋白水平。 |
