| 货号 | 2196T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | ESET (C1C12) Rabbit mAb detects endogenous levels of total ESET protein. The antibody does not cross-react with other SET-domain containing histone methyltransferase proteins. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). ESET also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters (3). ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits ESET to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits ESET to silenced promoters in cancer cells (7). ESET may play a role in the pathogenesis of Huntingtons disease, since levels of ESET protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
| 参考文献 | 1 . Yang, L. et al. (2002) Oncogene 21, 148-152. 2 . Wang, H. et al. (2003) Mol. Cell 12, 475-487. 3 . Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932. 4 . Yang, L. et al. (2003) Biochem. J. 369, 651-657. 5 . Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605. 6 . Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935. 7 . Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500. 8 . Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181. |
Confocal immunofluorescent analysis of MCF-7 cells using ESET (C1C12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用ESET (C1C12) Rabbit mAb (绿色)，共聚焦免疫荧光分析MCF-7细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of cell lysates from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb. 使用ESET (C1C12) Rabbit mAb，免疫印迹(Western blot)分析MCF7和293细胞中ESET (C1C12)的蛋白水平。 |
