| 货号 | 2207T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Rat,Zebrafish, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Shh (C9C5) Rabbit mAb detects the endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 19, (45 kDa precursor) |
| 参考文献 | 1 . Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87. 2 . McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114. 3 . Zhang, X.M. et al. (2001) Cell 106, 781-92. 4 . Adolphe, C. et al. (2004) Development 131, 5009-19. 5 . Pathi, S. et al. (2001) Mech Dev 106, 107-17. 6 . Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394. |
Western blot analysis of extracts from HT29 and GH3 cells, and GH3 cell conditioned medium (CM), using Shh (C9C5) Rabbit mAb. 使用Shh（C9C5）Rabbit mAb兔单抗对HT29和GH3细胞以及GH3细胞条件培养基（CM），进行western blot分析。 |
