| 货号 | 9239T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Bad (D24A9) Rabbit mAb detects endogenous levels of total Bad protein. The antibody does not cross-react with related proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
| 参考文献 | 1 . Yang, E. et al. (1995) Cell 80, 285-291. 2 . Zha, J. et al. (1996) Cell 87, 619-628. 3 . Datta, S.R. et al. (1997) Cell 91, 231-241. 4 . Peso, L. et al. (1997) Science 278, 687-689. 5 . Bonni, A. et al. (1999) Science 286, 1358-1362. 6 . Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867. 7 . Harada, H. et al. (1999) Mol. Cell 3, 413-422. 8 . Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869. 9 . Lizcano, J. et al. (2000) Biochem. J. 349, 547-557. 10 . Datta, S. et al. (2000) Mol. Cell 6, 41-51. |
Western blot analysis of extracts from various cell lines using Bad (D24A9) Rabbit mAb. Western免疫印迹。用 Bad (D24A9) Rabbit mAb 兔单抗研究各种细胞系的细胞提取液。 |
