A20/TNFAIP3 (D13H3) Rabbit mAb【科瑞奥博】

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A20/TNFAIP3 (D13H3) Rabbit mAb

货号： 5630T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	5630T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit IgG
应用	WB, IP
目标/特异性	A20/TNFAIP3 (D13H3) Rabbit mAb recognizes endogenous levels of total A20/TNFAIP3 protein.
使用方法	WB(1:1000) IP (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	A20, also referred to as TNF-α-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-κB (1,2). It was first identified as a TNF-α inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs (3). Constitutive expression of A20 is observed in lymphoid tissues (4), but it is transiently expressed in a variety of cell types in response to inflammatory signals such as TNF-α (3,5), IL-1 (3,5), phorbol esters (6), and LPS (7). Expression of A20 can confer resistance to apoptosis and NF-κB activation triggered by these signals, probably through interference with TRAF (TNF receptor associated factor) family members (8,9), and interaction with the NF-κB inhibiting protein ABIN (10). Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions (11,12). The amino-terminus of A20 contains de-ubiquitinating (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation (12).
存放说明	-20C
计算分子量	82
参考文献	1 . Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51. 2 . Lee, E.G. et al. (2000) Science 289, 2350-4. 3 . Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8. 4 . Tewari, M. et al. (1995) J Immunol 154, 1699-706. 5 . Jäättelä, M. et al. (1996) J Immunol 156, 1166-73. 6 . Laherty, C.D. et al. (1993) J Biol Chem 268, 5032-9. 7 . Hu, X. et al. (1998) Blood 92, 2759-65. 8 . Song, H.Y. et al. (1996) Proc Natl Acad Sci USA 93, 6721-5. 9 . Heyninck, K. and Beyaert, R. (1999) FEBS Lett 442, 147-50. 10 . Heyninck, K. et al. (1999) J Cell Biol 145, 1471-82. 11 . Evans, P.C. et al. (2004) Biochem J 378, 727-34. 12 . Lin, S.C. et al. (2008) J Mol Biol 376, 526-40.

参考图片

Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with a Myc-DDK-tagged human A20/TNFAIP3 construct (+), using A20/TNFAIP3 (D13H3) Rabbit mAb.Western免疫印迹。用A20/TNFAIP3 (D13H3) Rabbit mAb研究对照转染(-) 和转染了具有Myc-DDK末端的人A20/TNFAIP3的质粒(+) COS-7细胞的细胞提取液。

Western blot analysis of extracts from G-361, JJN-3, and A20 cell lines using A20/TNFAIP3 (D13H3) Rabbit mAb.Western免疫印迹。用A20/TNFAIP3 (D13H3) Rabbit mAb研究G-361, JJN-3 和 A20 细胞的细胞提取液。

Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 (40 nM) and A23187 (2 μM) for various amounts of time, using A20/TNFAIP3 (D13H3) Rabbit mAb.Western免疫印迹。用A20/TNFAIP3 (D13H3) Rabbit mAb研究未经处理的和经TPA #4174 (40 nM) 和A23187 (2 μM) 处理不同时间的Jurkat细胞的细胞提取液。