| 货号 | 5643T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Presenilin 1 (D39D1) Rabbit mAb recognizes endogenous levels of total Presenilin 1 protein. The antibody may detect a nonspecific band at 60 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilins associated γ-secretase activity for amyloid-β protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 22 (CTF) |
| 参考文献 | 1 . Haass, C. and De Strooper, B. (1999) Science 286, 916-9. 2 . Kimberly, W.T. et al. (2000) J. Biol. Chem. 275, 3173-3178. 3 . Kim, T.W. et al. (1997) J. Biol. Chem. 272, 11006-11010. |
Western blot analysis of extracts from HeLa, wild-type MEF, and Presenilin 1(-/-) MEF cells using Presenilin 1 (D39D1) Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (Wild-type MEF and Presenilin 1(-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium). Western blot分析HeLa、野生型和Presenilin 1(-/-) 突变型MEF细胞提取物，使用的抗体是Presenilin 1 (D39D1) Rabbit mAb 兔单抗（上图）及α/β-Tubulin Antibody 兔多抗#2148（下图）。（野生型MEF和Presenilin 1(-/-) 突变型MEF细胞由Dr.Bart De Strooper, K.U.Leuven, Belgium友情提供）。 |
