| 货号 | 2224T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB |
| 目标/特异性 | Caspase-2 (C2) Mouse mAb detects endogenous levels of procaspase-2 as well as its 14 and 12 kDa cleaved fragments. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-2 (Nedd2/ICH-1) is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved at Asp316, producing a 14 kDa fragment and a 32 kDa prodomain/large subunit. Subsequent processing at Asp152 and Asp330 produces an 18 kDa large subunit and a 12 kDa small fragment (1). Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD (2). This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events (3, 4). In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase (5). |
| 存放说明 | -20C |
| 计算分子量 | 12, 14, 48 |
| 参考文献 | 1 . Li, H. et al. (1997) J Biol Chem 272, 21010-7. 2 . Tinel, A. and Tschopp, J. (2004) Science 304, 843-6. 3 . Dirsch, V. M. et al. (2004) Oncogene 23, 1586-1593. 4 . Castedo, M. et al. (2004) Oncogene 23, 4362-70. 5 . Paroni, G. et al. (2001) J Biol Chem 276, 21907-15. |
Western Blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (5 hrs, 25uM), using Caspase-2 (C2) Mouse mAb. Western blot 检测Jurkat细胞提取物，未处理 (-)或 etoposide (依托泊甙)处理 (5 hrs, 25uM)，使用抗体为 Caspase-2 (C2) Mouse mAb 鼠单抗。 |
