| 货号 | 9746T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB, IP |
| 目标/特异性 | Caspase-8 (1C12) Mouse mAb detects endogenous levels of full length caspase-8 (57 kDa), the cleaved intermediate p43/p41 and the caspase-8 active fragment p18. This antibody does not cross-react with other caspases. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. |
| 存放说明 | -20C |
| 计算分子量 | 18, 43, 57 |
| 参考文献 | 1 . Muzio, M. et al. (1996) Cell 85, 817-27. 2 . Boldin, M.P. et al. (1996) Cell 85, 803-15. 3 . Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9. |
Western blot analysis of extracts from SKW6.4 cells, untreated or anti-Fas-treated (1 µg/ml), and Jurkat cells, untreated or etoposide-treated (25 µM), using Caspase-8 (1C12) Mouse mAb. Western blot 检测SKW6.4细胞系提取物，未处理或抗Fas抗体 (1 µg/ml)处理；WB检测 Jurkat 细胞提取物，未处理或etoposide（依托泊苷）(25 µM)处理，所用抗体为Caspase-8 (1C12) Mouse mAb 鼠单抗。 |
