| 货号 | 5845T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Toll-like Receptor 9 (D2C9) Rabbit mAb recognizes endogenous levels of total TLR9 protein. This antibody is predicted to react with known full-length isoforms of TLR9, but not with the cleaved TLR9 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm. |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Akira, S. (2003) J Biol Chem 278, 38105-8. 2 . Beutler, B. (2004) Nature 430, 257-63. 3 . Dunne, A. and ONeill, L.A. (2003) Sci STKE 2003, re3. 4 . Medzhitov, R. et al. (1997) Nature 388, 394-7. 5 . Schwandner, R. et al. (1999) J Biol Chem 274, 17406-9. 6 . Takeuchi, O. et al. (1999) Immunity 11, 443-51. 7 . Alexopoulou, L. et al. (2001) Nature 413, 732-8. 8 . Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4. 9 . Horng, T. et al. (2001) Nat Immunol 2, 835-41. 10 . Oshiumi, H. et al. (2003) Nat Immunol 4, 161-7. 11 . Muzio, M. et al. (1997) Science 278, 1612-5. 12 . Wesche, H. et al. (1997) Immunity 7, 837-47. 13 . Suzuki, N. et al. (2002) Nature 416, 750-6. 14 . Irie, T. et al. (2000) FEBS Lett 467, 160-4. 15 . Chuang, T.H. and Ulevitch, R.J. (2000) Eur Cytokine Netw 11, 372-8. 16 . Du, X. et al. (2000) Eur Cytokine Netw 11, 362-71. 17 . Hemmi, H. et al. (2000) Nature 408, 740-5. 18 . Bauer, S. et al. (2001) Proc Natl Acad Sci U S A 98, 9237-42. 19 . Takeshita, F. et al. (2001) J Immunol 167, 3555-8. 20 . Latz, E. et al. (2004) Nat Immunol 5, 190-8. 21 . Park, B. et al. (2008) Nat Immunol 9, 1407-14. 22 . Ewald, S.E. et al. (2008) Nature 456, 658-62. 23 . Sepulveda, F.E. et al. (2009) Immunity 31, 737-48. |
Western blot analysis of extracts from various cell lines using Toll-like Receptor 9 (D2C9) Rabbit mAb.Western免疫印迹。用Toll-like Receptor 9 (D2C9) Rabbit mAb研究各种细胞的细胞提取液。 |
