| 货号 | 8028T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | TRAF6 (D21G3) Rabbit mAb recognizes endogenous levels of total TRAF6 protein. This antibody is not predicted to cross-react with other TRAF family members. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system (5).TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling (6) as well as defects in neuronal development (7). Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities (8) that result in signaling responses from the interleukin-1 receptor (IL-1R) (9), toll-like receptor (10,11), CD40 (12), RANK (13,14), and p75 neurotrophin receptor (15). TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK (10). This leads to activation of NF-κB and MAP kinase signaling pathways through downstream association with the TAB/TAK-1 complex (16). TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation (17). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Arch, R.H. et al. (1998) Genes Dev 12, 2821-30. 2 . Chung, J.Y. et al. (2002) J Cell Sci 115, 679-88. 3 . Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91. 4 . Rothe, M. et al. (1994) Cell 78, 681-92. 5 . Cao, Z. et al. (1996) Nature 383, 443-6. 6 . Wu, H. and Arron, J.R. (2003) Bioessays 25, 1096-105. 7 . Darnay, B.G. et al. (1998) J Biol Chem 273, 20551-5. 8 . Lomaga, M.A. et al. (1999) Genes Dev 13, 1015-24. 9 . Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 10 . Wong, B.R. et al. (1999) Mol Cell 4, 1041-9. 11 . Lomaga, M.A. et al. (2000) J Neurosci 20, 7384-93. 12 . Ye, H. et al. (2002) Nature 418, 443-7. 13 . Muzio, M. et al. (1997) Science 278, 1612-5. 14 . Ishida, T. et al. (1996) J Biol Chem 271, 28745-8. 15 . Wong, B.R. et al. (1998) J Biol Chem 273, 28355-9. 16 . Khursigara, G. et al. (1999) J Biol Chem 274, 2597-600. 17 . Medzhitov, R. et al. (1998) Mol Cell 2, 253-8. |
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a cDNA expression construct encoding full-length human TRAF6 (+), using TRAF6 (D21G3) Rabbit mAb. Western blot方法检测293T细胞提取物，空质粒(-)或cDNA表达载体编码的全长人TRAF6转染(+)；使用抗体为TRAF6 (D21G3) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from various cell lines using TRAF6 (D21G3) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为TRAF6 (D21G3) Rabbit mAb 兔单抗。 |
