| 货号 | 2789T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Adiponectin (C45B10) Rabbit mAb detects endogenous levels of total adiponectin protein monomer. It will not detect higher molecular weight forms of adiponectin. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Adiponectin, also termed AdipoQ, Acrp30, apM1 and GBP28, is an adipokine expressed exclusively in brown and white adipocytes (1). It is secreted into the blood and exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer (1). Adiponectin levels are decreased in obese and insulin-resistant mice and humans (2), suggesting that this adipokine is critical to maintain insulin sensitivity. Adiponectin stimulates the phosphorylation of AMPKα at Thr172 and activates AMPK in skeletal muscle (3). It also stimulates glucose uptake in myocytes (3). The block of AMPK activation by a dominant-negative AMPKα2 isoform inhibits the effect of adiponectin on glucose uptake, indicating that adiponectin stimulates glucose uptake and increases insulin sensitivity through its action on AMPK (3). Adiponectin mutants that are not able to form oligomers larger than trimers have no effect on the AMPK pathway (4). Mutations that render adiponectin unable to form high molecular weight multimers are associated with human diabetes (4), indicating the importance of multimerization for adiponectin activity. |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | 1 . Kadowaki, T. et al. (2006) J Clin Invest 116, 1784-92. 2 . Hu, E. et al. (1996) J Biol Chem 271, 10697-703. 3 . Yamauchi, T. et al. (2002) Nat. Med. 8, 1288-1295. 4 . Waki, H. et al. (2003) J. Biol. Chem. 278, 40352-40363. |
Western blot analysis of extracts from NIH/3T3 and 3T3-L1 cells using Adiponectin (C45B10) Rabbit mAb. 对NIH/3T3和3T3-L1细胞裂解液使用Adiponectin (C45B10) Rabbit mAb兔单抗进行Western blot分析。 |
