| 货号 | 8193T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | ZO-1 (D7D12) Rabbit mAb recognizes endogenous levels of total ZO-1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and -2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. 2 . Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11. 3 . Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47. 4 . Umeda, K. et al. (2006) Cell 126, 741-54. 5 . Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66. 6 . Traweger, A. et al. (2003) J Biol Chem 278, 2692-700. 7 . Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36. 8 . Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24. |
Immunoprecipitation and western blot analysis of extracts from Hep G2 cells using ZO-1 (D7D12) Rabbit mAb. Lane 1 is 10% input. 使用ZO-1 (D7D12) Rabbit mAb兔单抗，免疫沉淀分析HepG2细胞中ZO-1蛋白。第1道是10% input。 | |
Western blot analysis of extracts from various cell lines using ZO-1 (D7D12) Rabbit mAb. 使用ZO-1 (D7D12) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析不同细胞中ZO-1 (D7D12)蛋白水平。 |
