| 货号 | 82630T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , F , IF-IC |
| 目标/特异性 | Sox9 (D8G9H) Rabbit mAb recognizes endogenous levels of total Sox9 protein. |
| 使用方法 | WB(1:1000) IHC-P (1:600) F (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sox9 is a transcription factor with an HMG-box DNA binding domain that has homology to the HMG domain of the mammalian testis-determining factor, SRY (1). Sox9 regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification (2,3). Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination (4). In addition, Sox9 is reportedly involved in the maintenance of adult stem cell populations, including multipotent neural stem cells (5), hair follicle stem cells (6), and mammary stem cells (7). Recent interest has focused on the role of Sox9 in tumor biology. For example, research studies have shown that Sox9 expression in lung adenocarcinoma induces a mesenchymal phenotype in tumor cells (8). Other research studies have shown that YAP1 induced upregulation of Sox9 confers cancer stem cell like properties on esophageal cancer cells (9). Moreover, Sox9 expression has been linked with several other tumor types including ovarian, prostate, and pancreatic malignancies (10-12). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Mertin, S. et al. (1999) Nucleic Acids Res 27, 1359-64. 2 . Akiyama, H. et al. (2002) Genes Dev 16, 2813-28. 3 . Raspopovic, J. et al. (2014) Science 345, 566-70. 4 . De Santa Barbara, P. et al. (1998) Mol Cell Biol 18, 6653-65. 5 . Scott, C.E. et al. (2010) Nat Neurosci 13, 1181-9. 6 . Kadaja, M. et al. (2014) Genes Dev 28, 328-41. 7 . Guo, W. et al. (2012) Cell 148, 1015-28. 8 . Capaccione, K.M. et al. (2014) Oncotarget 5, 3636-50. 9 . Song, S. et al. (2014) Cancer Res 74, 4170-82. 10 . Raspaglio, G. et al. (2014) Gene 542, 173-81. 11 . Qin, G.Q. et al. (2014) Onco Targets Ther 7, 137-46. 12 . Shroff, S. et al. (2014) Hum Pathol 45, 456-63. |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Sox9 (D8G8H) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Sox9 (D8G8H) Rabbit mAb. | |
Flow cytometric analysis of HeLa cells using an isotype control (red) and Sox9 (D8G8H) Rabbit mAb (blue) and A-204 cells using Sox9 (D8G8H) Rabbit mAb (green). Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Sox9 (D8G8H) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Sox9 (D8G8H) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells are negative for Sox9. | |
Immunohistochemical analysis of paraffin-embedded A-204 (left) and U-87 MG (right) cell pellets using Sox9 (D8G8H) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse testis using Sox9 (D8G8H) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Confocal immunofluorescent analysis of A-204 (left) and HeLa (right) cells using Sox9 (D8G8H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). |
