| 货号 | 2679T |
| 同种亚型 | Rabbit |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IHC-P , IF-IC |
| 目标/特异性 | Calnexin (C5C9) Rabbit mAb detects endogenous levels of total calnexin protein. |
| 使用方法 | WB(1:1000) IHC-P (1:400) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. Calnexin was first identified as being involved in the assembly of murine class I histocompatibility molecules (1,2). Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Degen, E. and Williams, D.B. (1991) J. Cell Biol. 112, 1099-1115. 2 . Ahluwalia, N. et al. (1992) J. Biol. Chem. 267, 10914-10918. 3 . Rajagopalan, S. et al. (1994) Science 263, 387-390. 4 . Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128. 5 . Williams, D.B. (2006) J. Cell Sci. 119, 615-623. |
Confocal immunofluorescent analysis of HeLa cells using Calnexin (C5C9) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用Calnexin (C5C9) Rabbit mAb（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。 | |
Western blot analysis of extracts from PANC1, HepG2 and A204 cells using Calnexin (C5C9) Rabbit mAb. 对PANC1、HepG2和A204细胞抽提液使用Calnexin (C5C9) Rabbit mAb兔单抗进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Calnexin (C5C9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人乳腺癌使用Calnexin (C5C9) Rabbit mAb兔单抗进行免疫组织化学分析，其中左图为加入对照肽，右图为加入抗原特异性肽。 |
