Oct-4A (D6C8T) Rabbit mAb (Mouse Specific)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Oct-4A (D6C8T) Rabbit mAb (Mouse Specific)

货号： 83932T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	83932T
同种亚型	Rabbit IgG
反应种属	Mouse,
来源宿主	Rabbit IgG
应用	WB, IP , F , IF-IC , ChIP
目标/特异性	Oct-4A (D6C8T) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total murine Oct-4A protein.
使用方法	WB(1:1000) IP (1:100) F (1:200) IF-IC (1:800) ChIP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).
存放说明	-20C
计算分子量	45
参考文献	1 . Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50. 2 . Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8. 3 . Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. 4 . Cheng, L. et al. (2007) J Pathol 211, 1-9. 5 . Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20. 6 . Cauffman, G. et al. (2006) Stem Cells 24, 2685-91. 7 . Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

参考图片

Flow cytometric analysis of NIH/3T3 cells (blue) and F9 cells (green) using Oct-4A (D6C8T) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab)₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ mES cells and either 10 μl of Oct-4A (D6C8T) Rabbit mAb (Mouse Specific) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^® Mouse Oct-4 Promoter Primers #4653, SimpleChIP^® Mouse XIST Intron 1 Primers #4659, and SimpleChIP^® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Confocal immunofluorescent analysis of F9 (upper) and NIH/3T3 (lower) cells using Oct-4A (D6C8T) Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western blot analysis of extracts from F9 and NIH/3T3 cells using Oct-4A (D6C8T) Rabbit mAb (Mouse Specific) (upper) and GAPDH (D16H11) XP^® Rabbit mAb #5174 (lower). As expected, NIH/3T3 cells do not express Oct-4A.

Immunoprecipitation of Oct-4A from F9 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is Oct-4A (D6C8T) Rabbit mAb (Mouse Specific). Western blot analysis was performed using Oct-4A (D6C8T) Rabbit mAb (Mouse Specific).