| 货号 | 2620T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Jagged1 (28H8) Rabbit mAb detects endogenous levels of total Jagged1 protein. It does not cross-react with Jagged2. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
| 参考文献 | 1 . Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868. 2 . Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328. 3 . Chiba, S. (2006) Stem Cells 24, 2437-2447. 4 . Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610. 5 . Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643. 6 . LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437. 7 . Li, L. et al. (1997) Nat. Genet. 16, 243-251. 8 . Röpke, A. et al. (2003) Hum. Mutat. 21, 100. 9 . Eldadah, Z.A. et al. (2001) Hum. Mol. Genet. 10, 163-169. 10 . Santagata, S. et al. (2004) Cancer Res 64, 6854-6857. |
Western blot analysis of total cell lysates from HepG2 and LNCaP cells, using Jagged1 (28H8) Rabbit mAb. 使用Jagged1 (28H8) 兔单抗对HepG2和LNCaP细胞全细胞提取物进行western blot分析。 |
