| 货号 | 12827T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Caspase-7 (D2Q3L) Rabbit mAb recognizes endogenous levels of total caspase-7 protein. This antibody detects both the full-length (35 kDa) and large (20 kDa) subunits following caspase-7 activation. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5). |
| 存放说明 | -20C |
| 计算分子量 | 20, 35 |
| 参考文献 | 1 . Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52. 2 . Duan, H. et al. (1996) J Biol Chem 271, 1621-5. 3 . Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8. 4 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 5 . Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11. |
Western blot analysis of extracts from Jurkat and A20 cells, untreated (-) or treated with Etoposide #2200 (25 μM, overnight; +), using Caspase-7 (D2Q3L) Rabbit mAb. Western blot 分析Jurkat 和 A20 细胞提取物，未处理（-）和Etoposide #2200 （25 μM，过夜；+）处理，使用抗体为 Caspase-7 (D2Q3L) Rabbit mAb 兔单抗。 |
