| 货号 | 8727T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey,D. melanogaster, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, F , IF-IC |
| 目标/特异性 | MEK1/2 (D1A5) Rabbit mAb recognizes endogenous levels of total MEK1 and MEK2 proteins. This antibody is predicted to cross-react with MEK1/MEK2 orthologs in a variety of species. |
| 使用方法 | WB(1:1000) F (1:400) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Crews, C.M. et al. (1992) Science 258, 478-480. 2 . Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19. 3 . Rosen, L.B. et al. (1994) Neuron 12, 1207-21. 4 . Cowley, S. et al. (1994) Cell 77, 841-52. |
Flow cytometric analysis of Jurkat cells using MEK1/2 (D1A5) Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). | |
Confocal immunofluorescent analysis of HeLa cells using MEK1/2 (D1A5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
