| 货号 | 8729T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | SUV39H1 (D11B6) Rabbit mAb recognizes endogenous levels of total SUV39H1 protein. This antibody does not cross-react with the SUV39H2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Human histone methyltransferase SUV39H1 is the homolog of the dominant Drosophila PEV modifier Su(var)3-9 and is composed of 412 amino acid residues (1). It combines two of the most evolutionarily conserved domains of the "chromatin regulators": the chromo and SET domains (2,3). The 60 amino acid chromo domain represents an ancient histone-like fold that directs heterochromatic localizations. It has been demonstrated that the 130 amino acid SET domain contains the methyltransferase catalytic motif, which cooperates with the adjacent cysteine-rich regions to confer histone methyltransferase activity (1). This enzyme selectively methylates histone H3 on Lys9, which generates a binding site for HP1 proteins, a family of heterochromatic adaptor molecules involved in both gene silencing and supra-nucleosomal chromatin structure (4,5). SUV39H1 histone methyltransferase plays an important role in modification of histone amino termini and regulation of gene expression. |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Rea, S. et al. (2000) Nature 406, 593-599. 2 . Koonin, E. V. et al. (1995) Nucleic Acids Res. 23, 4229-4232. 3 . Jenuwein, T. et al. (1998) Cell. Mol. Life Sci. 54, 80-93. 4 . Lachner, M. et al. (2001) Nature 410, 116-120. 5 . Bannister, A. J. et al. (2001) Nature 410, 120-123. |
Western blot analysis of extracts from various cell lines using SUV39H1 (D11B6) Rabbit mAb. 使用SUV39H1 (D11B6) Rabbit mAb，免疫印迹(Western blot)分析不同细胞中SUV39H1 (D11B6)的蛋白水平。 |
