c-Jun (60A8) Rabbit mAb【科瑞奥博】

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c-Jun (60A8) Rabbit mAb

货号： 9165T 基本售价： 1350.0 元规格： -

产品信息

概述
货号	9165T
同种亚型	Rabbit IgG
反应种属	Human,Mouse,Rat,Monkey,
来源宿主	Rabbit IgG
应用	WB, IP , IHC-P , IF-IC , IHC-F , ChIP
目标/特异性	c-Jun (60A8) Rabbit mAb detects endogenous levels of total c-Jun protein, regardless of phosphorylation state.
使用方法	WB(1:1000) IP (1:50) IHC-P (1:400) IF-IC (1:50) IHC-F (1:400) ChIP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).
存放说明	-20C
计算分子量	43, 48
参考文献	1 . Jochum, W. et al. (2001) Oncogene 20, 2401-12. 2 . Davis, R.J. (2000) Cell 103, 239-52. 3 . Hilberg, F. et al. (1993) Nature 365, 179-81. 4 . Raivich, G. et al. (2004) Neuron 43, 57-67. 5 . Behrens, A. et al. (2002) EMBO J 21, 1782-90. 6 . Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700. 7 . Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. 8 . Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6. 9 . Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3. 10 . Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20. 11 . Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81. 12 . Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

参考图片

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of c-Jun (60A8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Immunohistochemical analysis of frozen H1650 xenograft, showing nuclear localization using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的冷冻H1650移植瘤组织，显示出细胞核的位置。使用的抗体为c-Jun (60A8) Rabbit mAb。

Confocal immunofluorescent analysis of HeLa cells, using c-Jun (60A8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞，使用的抗体是c-Jun (60A8) Rabbit mAb (绿色)。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记，呈红色。

Immunohistochemical analysis of paraffin-embedded human astrocytoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人星形细胞瘤组织，使用的抗体为c-Jun (60A8) Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人卵巢癌组织，使用的抗体为c-Jun (60A8) Rabbit mAb。

Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells, untreated or UV-treated, using c-Jun (60A8) Rabbit mAb.Western blot检测细胞提取物：未经处理和用紫外照射的NIH/3T3 和SK-N-MC 细胞，是用的抗体是c-Jun (60A8) Rabbit mAb。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using c-Jun (60A8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人结肠癌组织，使用的抗体为c-Jun (60A8) Rabbit mAb。