| 货号 | 4656T |
| 同种亚型 | Mouse IgE |
| 反应种属 | Human, |
| 来源宿主 | Mouse IgE |
| 应用 | WB, F |
| 目标/特异性 | Cyclin A2 (BF683) Mouse mAb detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1. |
| 使用方法 | WB(1:2000) F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of over-expressed cyclin A to accellerate the G1 to S transition causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).通过G1/S期检验点后开始DNA复制需要周期蛋白E的参与，而周期蛋白B参与了G2/M期检验点之后有丝分裂过程的启动，周期蛋白A似乎在S期和M期都起作用(1)。许多研究显示过表达周期蛋白A能够加速G1到S期的过渡，引起DNA复制；而周期蛋白A的反义DNA可以阻止DNA复制(2-4)。周期蛋白A的作用显然是进入有丝分裂的限速步骤，同时参与分裂前期的完成(5)。分裂前期的晚期，因为cdc2/cyclinB1开始活跃，周期蛋白A不再必要(5)。 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Pagano, M. et al. (1992) EMBO J 11, 961-71. 2 . Resnitzky, D. et al. (1995) Mol. Cell. Biol. 15, 4347-4352. 3 . dUrso, G. et al. (1990) Science 250, 786-791. 4 . Zindy, F. et al. (1992) Biochem. Biophys. Res. Commun. 182, 1144-1154. 5 . Furuno, N. et al. (1999) J. Cell. Biol. 147, 295-306. |
Flow cytometric analysis of untreated Jurkat cells, using Cyclin A (BF683) Mouse mAb versus propidium iodide (DNA content). Note decrease in expression of Cyclin A during late mitosis (arrow). 流式细胞术分析未处理的Jurkat细胞。使用的抗体为 Cyclin A (BF683) Mouse mAb ，DNA内容物使用碘化丙啶染色。在有丝分裂晚期可见周期蛋白A表达减少（如箭头所示）。 | |
Western analysis of extracts from Hela cells that were untreated, treated with doxorubicin (0.5 µM, 24 hours), or with nocodazole (50 ng/ml, 24 hours), using Cyclin A2 (BF683) Mouse mAb. |
