| 货号 | 9355T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey,Guinea Pig, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | RSK1/RSK2/RSK3 (32D7) Rabbit mAb detects endogenous levels of total RSK1/RSK2/RSK3 proteins. |
| 使用方法 | WB(1:1000) IP (1:25) IF-IC (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9. 2 . Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8. 3 . Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. |
Confocal immunofluorescent analysis of HeLa cells labeled with RSK1/RSK2/RSK3 (32D7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞，标记RSK1/RSK2/RSK3 (32D7) Rabbit mAb（呈绿色）。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记，呈红色。 | |
Western blot analysis of extracts from HeLa, L929 and AR42J cells, using RSK1/RSK2/RSK3 (32D7) Rabbit mAb.Western blot方法检测细胞提取物：HeLa, L929，AR42J细胞。使用的抗体是RSK1/RSK2/RSK3 (32D7) Rabbit mAb。 |
