| 货号 | 8969T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Apaf-1 (D5C3) Rabbit mAb recognizes endogenous levels of total Apaf-1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 135 |
| 参考文献 | 1 . Zou, H. et al. (1997) Cell 90, 405-13. 2 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 3 . Saleh, A. et al. (1999) J Biol Chem 274, 17941-5. 4 . Benedict, M.A. et al. (2000) J Biol Chem 275, 8461-8. 5 . Cecconi, F. et al. (1998) Cell 94, 727-37. 6 . Yoshida, H. et al. (1998) Cell 94, 739-50. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Apaf-1 siRNA I #6229 (+), using Apaf-1 (D5C3) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Apaf-1 (D5C3) Rabbit mAb confirms silencing of Apaf-1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 免疫印迹分析转染了100 nM SignalSilence® Control siRNA (未偶联) #6568 (-) 或者 SignalSilence® Apaf-1 siRNA I #6229 (+)的HeLa细胞的细胞提取液，用抗体Apaf-1 (D5C3) Rabbit mAb 兔单抗(上图)或者α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 (下图)研究。Apaf-1 (D5C3) Rabbit mAb 兔单抗证实对Apaf-1表达的沉默, α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样量的对照。 | |
Western blot analysis of extracts from various cell lines using Apaf-1 (D5C3) Rabbit mAb. 用 Apaf-1 (D5C3) Rabbit mAb 兔单抗对各种细胞系的细胞提取液进行 Western blot （免疫印记）。 |
