| 货号 | 9582T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IHC-F |
| 目标/特异性 | β-Catenin (6B3) Rabbit mAb detects endogenous levels of total β-catenin. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) IHC-F (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | -20C |
| 计算分子量 | 92 |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90. |
Immunohistochemical analysis of frozen HCC-827 xenograft using beta-Catenin (6B3) Rabbit mAb. 使用 β-Catenin (6B3)兔单抗对冷冻的HCC-827爪蟾进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (6B3) Rabbit mAb. 使用 β-Catenin (6B3)兔单抗对石蜡包埋的人肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using β-Catenin (6B3) Rabbit mAb. 使用 β-Catenin (6B3)兔单抗对石蜡包埋的人肝细胞癌组织进行免疫组化分析。 | |
Western blot analysis of total cell lysates from 293, Rat2 and COS cells, using β-Catenin (6B3) Rabbit mAb. 使用 β-Catenin (6B3)兔单抗对293，Rat2和COS细胞全细胞提取物进行western blot分析。 |
