| 货号 | 9585T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | Slug (C19G7) Rabbit mAb detects endogenous levels of total Slug protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in γ-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Inukai, T. et al. (1999) Mol Cell 4, 343-52. 2 . Bolós, V. et al. (2003) J Cell Sci 116, 499-511. 3 . Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31. 4 . Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61. 5 . Castro Alves, C. et al. (2007) J Pathol 211, 507-15. 6 . Wu, W.S. et al. (2005) Cell 123, 641-53. 7 . Pérez-Mancera, P.A. et al. (2006) Cytogenet Genome Res 114, 24-9. |
Confocal immunofluorescent analysis of A204 cells (left) and PANC-1 cells (right) using Slug (C19G7) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).使用Slug（C19G7）兔单抗（绿色）对A204细胞（左）和PANC-1细胞（右）进行激光共聚焦荧光分析。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。 | |
Western blot analysis of extracts from A204, SKMEL5, and NIH/3T3 cells using Slug (C19G7) Rabbit mAb.使用Slug（C19G7）兔单抗对A204，SKMEL5和NIH/3T3细胞提取物进行western blot分析。 |
