| 货号 | 9616T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | PKCδ (D10E2) Rabbit mAb recognizes endogenous levels of total PKCδ protein. This antibody does not cross-react with other PKC isoforms. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
| 参考文献 | 1 . Nishizuka, Y. (1984) Nature 308, 693-8. 2 . Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403. 3 . Mellor, H. and Parker, P.J. (1998) Biochem J 332 ( Pt 2), 281-92. 4 . Ron, D. and Kazanietz, M.G. (1999) FASEB J 13, 1658-76. 5 . Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep 1, 399-403. 6 . Baron, C.L. and Malhotra, V. (2002) Science 295, 325-8. 7 . Flynn, P. et al. (2000) J Biol Chem 275, 11064-70. 8 . Lu, Z. et al. (1997) Mol Cell Biol 17, 3418-28. 9 . Benes, C. and Soltoff, S.P. (2001) Am J Physiol Cell Physiol 280, C1498-510. 10 . Li, W. et al. (1997) J Biol Chem 272, 24550-5. 11 . Le Good, J.A. et al. (1998) Science 281, 2042-5. 12 . Morita, M. et al. (2008) J Biochem 143, 31-8. 13 . Denning, M.F. et al. (1996) J Biol Chem 271, 5325-31. 14 . Kronfeld, I. et al. (2000) J Biol Chem 275, 35491-8. |
Western blot analysis of bacterially expressed, GST-tagged, purified PKC isoforms, using PKCδ (D10E2) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower), demonstrating specificity for PKCδ. Western blot方法检测细菌表达，用GST-标记纯化的PKC亚型以验证PKCδ的特异性，使用的抗体为PKCδ (D10E2) Rabbit mAb (上图)或 GST (91G1) Rabbit mAb #2625 (下图). | |
Western blot analysis of extracts from various cell lines using PKCδ (D10E2) Rabbit mAb. Western blot方法检测多个细胞系的提取物，使用的抗体为PKCδ (D10E2) Rabbit mAb. |
