| 货号 | 9644T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | 4E-BP1 (53H11) Rabbit mAb detects endogenous levels of total 4E-BP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:2400) F (1:1600) IF-IC (1:1600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15-20 |
| 参考文献 | 1 . Pause, A. et al. (1994) Nature 371, 762-7. 2 . Brunn, G.J. et al. (1997) Science 277, 99-101. 3 . Gingras, A.C. et al. (1998) Genes Dev 12, 502-13. 4 . Fadden, P. et al. (1997) J Biol Chem 272, 10240-7. 5 . Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37. |
Immunohistochemical analysis of paraffin-embedded mouse spleen, 4E-BP1/2 wild type (left) or 4E-BP1 knockout (right), using 4E-BP1 (53H11) Rabbit mAb. 4E-BP wild type and knockout tissues kindly provided by Dr. Nahum Sonenberg, McGill University. 使用4E-BP1 (53H11) Rabbit mAb兔单抗，免疫组化分析小鼠脾脏组织石蜡切片，切片分为4E-BP1/2野生型(左图)和4E-BP1敲除(右图)。Dr. Nahum Sonenberg（McGill University）友好的提供了4E-BP野生型和敲除的组织。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 4E-BP1 (53H11) Rabbit mAb in the presence of control peptide (left) or 4E-BP1 blocking peptide #1053 (right). 使用4E-BP1 (53H11) Rabbit mAb兔单抗免疫组化分析人类乳腺癌石蜡切片，切片分别孵育对照多肽（左图）或4E-BP1 blocking peptide #1053（右图）。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using 4E-BP1 (53H11) Rabbit mAb. 使用4E-BP1 (53H11) Rabbit mAb兔单抗，免疫组化分析人类肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using 4E-BP1 (53H11) Rabbit mAb. 使用4E-BP1 (53H11) Rabbit mAb，免疫组化分析人类肝癌组织石蜡切片。 | |
Western blot analysis of extracts from various cell lines using 4E-BP1 (53H11) Rabbit mAb. 使用4E-BP1 (53H11) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析在不同种细胞系提取物。 | |
Confocal immunofluorescent analysis of HeLa cells using 4E-BP1 (53H11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of COS cells using 4E-BP1 (53H11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). |
