| 货号 | 2520T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | SUFU (C54G2) Rabbit mAb detects endogenous level of total SUFU protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SUFU (Suppressor of Fused) was identified in Drosophila as a suppressor of the Fused (Fu) kinase that is essential for Hedgehog signaling during embryonic pattern formation (1). SUFU suppresses Hedgehog signaling by regulating the localization of the transcription factors Gli and Ci (2,3). In Drosophila, SUFU may also positively regulate Hedgehog signaling depending on SUFU protein levels and Hedgehog signal intensity (4). SUFU may function as a tumor suppressor as inactivation and loss of heterozygosity of SUFU is associated with human rhabdomyosarcomas and medulloblastomas (5,6). Deletion of SUFU in mice results in embryonic lethality, while heterozygotes exhibit developmental defects characteristic of basal cell nevus syndrome. This aberrant developmental pathway is attributed to ligand-independent activation of Hedgehog signaling (7). GSK-3β binds and phosphorylates SUFU in vitro and additional information predicts that GSK-3β may positively regulate Hedgehog signaling through modification of SUFU (8). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Pham, A. et al. (1995) Genetics 140, 587-98. 2 . Barnfield, P.C. et al. (2005) Differentiation 73, 397-405. 3 . Méthot, N. and Basler, K. (2000) Development 127, 4001-10. 4 . Dussillol-Godar, F. et al. (2006) Dev Biol 291, 53-66. 5 . Tostar, U. et al. (2006) J. Pathol. 208, 17-25. 6 . Taylor, M.D. et al. (2002) Nat. Genet. 31, 306-310. 7 . Svärd, J. et al. (2006) Dev. Cell. 10, 187-197. 8 . Takenaka, K. et al. (2007) Biochem. Biophys. Res. Commun. 353, 501-508. |
Western blot analysis of total cell lysates from various cell types using SUFU (C54G2) Rabbit mAb. 使用SUFU (C54G2) 兔单抗对多种细胞全细胞提取物进行western blot分析。 |
