| 货号 | 2468T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | PTCH1 (C53A3) Rabbit mAb detects transfected levels of PTCH1. This antibody can also detect endogenous levels of PTCH1 through immunoprecipitation and followed by Western blot analysis. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 180-210 |
| 参考文献 | 1 . Stone, D.M. et al. (1996) Nature 384, 129-34. 2 . Chen, Y. and Struhl, G. (1996) Cell 87, 553-63. 3 . Motoyama, J. et al. (1998) Nat Genet 18, 104-6. 4 . Smyth, I. et al. (1999) Hum Mol Genet 8, 291-7. 5 . Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87. 6 . McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114. 7 . Hahn, H. et al. (1996) Cell 85, 841-851. 8 . Johnson, R.L. et al. (1996) Science 272, 1668-1671. 9 . Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201. 10 . Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157. 11 . Watkins, D.N. et al. (2003) Nature 422, 313-317. 12 . Karhadkar, S.S. et al. (2004) Nature 431, 707-712. 13 . Agren, M. et al. (2004) Gene 330, 101-114. 14 . Berman, D.M. et al. (2003) Nature 425, 846-851. |
Western blot analysis of total cell lysates from COS cells, untransfected or transiently transfected with a human PTCH1 expression construct, using PTCH1 (C53A3) Rabbit mAb. 未转染或瞬转了人PTCH1表达载体的COS细胞，使用PTCH1 (C53A3)兔单抗对全细胞裂解物进行western blot分析。 | |
Immunoprecipitation of endogenous PTCH1 from 293 cell lysates using PTCH1 (C53A3) Rabbit mAb. Western blot detection was performed using the same antibody. 使用PTCH1 (C53A3) 兔单抗对293细胞内源性PTCH1进行免疫沉淀。使用同样的抗体进行western blot分析。 |
