| 货号 | 25229T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse, Rat |
| 应用 | WB,IHC |
| 目标/特异性 | CD4 (D7D2Z) Rabbit mAb recognizes endogenous levels of total mouse and rat CD4 protein. Non-specific staining in mouse kidney and liver has been observed. |
| 使用方法 | Western Blotting (1:1000) IHC-Leica® Bond™ (1:100) Immunohistochemistry (Paraffin) (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation (1). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Zamoyska, R. (1994) Immunity 1, 243-46. |
Western blot analysis of extracts from various cell lines and tissues using CD4 (D7D2Z) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using CD4 (D7D2Z) Rabbit mAb performed on the Leica® BOND™ Rx. | |
Immunohistochemical analysis of paraffin-embedded mouse colon using CD4 (D7D2Z) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD4 (D7D2Z) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD4 (D7D2Z) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded rat lung using CD4 (D7D2Z) Rabbit mAb. |
