| 货号 | 12790T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IHC-P , F , IF-IC |
| 目标/特异性 | CDK4 (D9G3E) Rabbit mAb recognizes endogenous levels of total CDK4 protein. In COS-7 cells, this antibody recognizes a 40 kDa background band of unknown origin. This antibody does not cross-react with CDK1, 2, or 6. |
| 使用方法 | WB(1:1000) IHC-P (1:800) F (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).细胞周期依赖激酶的活性受以下因素调控，包括T-loop磷酸化（CDK4的172位苏氨酸），其周期伴侣蛋白的丰度，和CDK抑制因子Cip/Kip 或INK家族蛋白的结合（1）。CDK4/cyclin D 和p27 Kip1/Cip1三聚体复合物的失活需要胞外有丝分裂原刺激以释放并降解p27，p27可以通过限制点和pRb-依赖的S期准入，影响细胞周期进程（2）。CDK4/cyclin D活性复合物可以调节城市网膜细胞瘤蛋白的磷酸化，促进E2F转录因子释放激活G1/S期基因表达（3）。在HeLa细胞中，收到UV辐射后，p16 INK4A上调表达伴随CDK4/cyclin D3引起G2期延迟，提示CDK4/cyclin D3在通过G2期过程中发挥作（4）。 |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81. 2 . Sherr, C.J. (1996) Science 274, 1672-7. 3 . Lukas, J. et al. (1996) Mol Cell Biol 16, 6917-25. 4 . Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9. |
Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb.使用CDK4 (D9G3E) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of MCF7 cells using CDK4 (D9G3E) Rabbit mAb (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8493 (red), and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #3458 (blue pseudocolor).使用CDK4 (D9G3E) Rabbit mAb (绿色)，p21 Waf1/Cip1 (12D1)Rabbit mAb (Alexa Fluor® 555 Conjugate) #8493 (红色)和Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #3458 (蓝色假色)对MCF7细胞进行激光共聚焦免疫荧光分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CDK4 (D9G3E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).使用CDK4 (D9G3E) Rabbit mAb对石蜡包埋的人乳腺癌组织进行进行免疫荧光分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK4 (D9G3E) Rabbit mAb.在对照肽（左）或抗原特异性肽段（右）的存在下，使用CDK4 (D9G3E) Rabbit mAb对石蜡包埋的人肺癌组织进行免疫荧光分析。 | |
Flow cytometric analysis of Jurkat cells using CDK4 (D9G3E) Rabbit mAb and Propidium Iodid (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
