| 货号 | 2366T |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | All Species Expected, |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB, IP |
| 目标/特异性 | His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
| 参考文献 | 1 . Kroll, D.J. et al. (1993) DNA Cell Biol 12, 441-53. 2 . di Guan, C. et al. (1988) Gene 67, 21-30. |
Western blot analysis of extracts from control (lane 1) or cells expressing C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb.Weatern blot分析对照组细胞提取物（泳道1），泳道2 为C末端表达His标签的蛋白，所用抗体为His-Tag (27E8) Mouse mAb. 。 | |
Immunoprecipitation of control lysate (lane 1) or C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb, followed by Western analysis with His-Tag Polyclonal Antibody #2365.免疫沉淀分析对照组细胞提取物（泳道1），泳道2 为C末端表达His标签的蛋白，所用抗体为His-Tag (27E8) Mouse mAb，采用His-Tag Polyclonal Antibody #2365进行western分析。 |
