| 货号 | 2764T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC , IHC-F |
| 目标/特异性 | Bcl-xL (54H6) Rabbit mAb detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:300) F (1:400) IF-IC (1:200) IHC-F (1:300) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Adams, J.M. and Cory, S. (1998) Science 281, 1322-6. 2 . Minn, A.J. et al. (1999) EMBO J 18, 632-43. 3 . Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9. 4 . Fan, M. et al. (2000) J Biol Chem 275, 29980-5. 5 . Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8. |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic localization, using Bcl-xL (54H6) Rabbit mAb. 免疫组化分析石蜡包埋的人前列腺癌组织，图示为细胞质的定位，所用抗体为 Bcl-xL (54H6) Rabbit mAb 兔单抗# 2764。 | |
Flow cytometric analysis of untreated Jurkat cells, using Bcl-xL (54H6) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 流式细胞仪分析未经处理的Jurkat 细胞，所用抗体为 Bcl-xL (54H6) Rabbit mAb 兔单抗(蓝色)，非特异性阴性对照抗体做比对(红色)。 | |
Western blot analysis of extracts from Jurkat and HeLa (human), COS (monkey), NIH/3T3 and L929 (mouse), and PC12 and C6 (rat) cells, using Bcl-xL (54H6) Rabbit mAb. Western blot 分析Jurkat和 HeLa (人源)、COS (猴子)、NIH/3T3 和 L929 (小鼠)、PC12 和 C6 (大鼠) 细胞的细胞提取液，用Bcl-xL (54H6) Rabbit mAb 兔单抗 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Bcl-xL (54H6) Rabbit mAb in the presence of control peptide (left) or Bcl-xL Blocking Peptide #1225 (right). 免疫组化分析石蜡包埋的人结肠癌组织，用对照多肽(左图) 或者 Bcl-xL Blocking Peptide #1225(右图)处理，所用抗体为 Bcl-xL (54H6) Rabbit mAb # 兔单抗2764。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Bcl-xL (54H6) Rabbit mAb. 免疫组化分析石蜡包埋的人肺癌组织，所用抗体为 Bcl-xL (54H6) Rabbit mAb 兔单抗# 2764。 | |
Immunoprecipitation of Bcl-xL from Jurkat cell extracts, using Bcl-xL (54H6) Rabbit mAb. Lane 1 is the lysate control, lane 2 is antibody alone and lane 3 is antibody plus lysate. 免疫共沉淀Jurkat细胞提取液中的 Bcl-xL 蛋白，所用抗体为 Bcl-xL (54H6) Rabbit mAb 兔单抗。泳道 1 是提取液，作为对照; 泳道 2是只有抗体 ，泳道3 是抗体加裂解液。 | |
Immunohistochemical analysis of paraffin-embedded 4TI syngeneic mouse tumor, using Bcl-xL (54H6) Rabbit mAb # 2764. 免疫组化分析石蜡包埋的4TI 同系小鼠肿瘤组织，所用抗体为 Bcl-xL (54H6) Rabbit mAb 兔单抗# 2764。 | |
Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Bcl-xL (54H6) Rabbit mAb. 免疫组织化学研究冰冻异种移植H1650，图示为细胞质的定位，所用抗体为Bcl-xL (54H6) Rabbit mAb 兔单抗。 |
