| 货号 | 2278T |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Myc-Tag (71D10) Rabbit mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. |
| 使用方法 | WB(1:1000) IP (1:200) F (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
| 存放说明 | -20C |
| 参考文献 | 1 . Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93. |
Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing Myc-Bcl-2 (+), using Bcl-2 Antibody #2872 (left) and Myc-Tag (71D10) Rabbit mAb (right).Western blot 分析未转染对照组（-）和转染过表达Myc-Bcl-2 (+)的细胞，所用抗体为Bcl-2 Antibody #2872 (左图) 和 Myc-Tag (71D10) Rabbit mAb (右图)。 | |
Immunocytochemical staining of cells expressing Myc-Bcl-2 protein (left) or cells without transfected protein (right), using Myc-tag (71D10) Rabbit mAb.免疫细胞化学分析Myc-Bcl-2的蛋白（上图），下图为未 转染的细胞，所用抗体为Myc-tag (71D10) Rabbit mAb。 | |
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag (71D10) Rabbit mAb.流式细胞术分选COS细胞，蓝色为未转染组，绿色为Myc转染的细胞，所用抗体为Myc-Tag (71D10) Rabbit mAb。 |
