| 货号 | 8954S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues. |
| 使用方法 | WB(1:2000) IP (1:200) F (1:400) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. |
| 存放说明 | -20C |
| 计算分子量 | N/A |
Confocal immunofluorescent analysis of C2C12 cells, serum-starved (left), treated with H2O2 (2 mM, 10 min; middle), or treated with H2O2 followed by λ phosphatase (right), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光术分别检测血清饥饿（左图）、 H2O2 (2 mM, 10 min; middle)处理或H2O2后再用λ磷酸酶处理（右图）的C2C12 细胞，使用的抗体为Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin标记（红色）。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。 | |
Flow cytometric analysis of K-562 cells, untreated (green) or Gleevec®-treated (blue), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb. 流式细胞术分别检测未处理（绿色）或经Gleevec®处理（蓝色）的K-562细胞活性，使用的抗体为Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb。 | |
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb. Western blot image was obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology). Western blot方法分别检测未处理（绿色）或经Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +)处理的A-431细胞提取物，使用的抗体为Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb。使用Odyssey® Infrared Imaging System (LI-COR® Biotechnology)进行western blot成像。 |
