CD1c (BDCA-1)+ DC Isolation Kit, human
货号：
130-090-506 基本售价：
询价 规格：
EA
产品信息
概述| 货号 | 130-090-506 |
| 描述 | The CD1c (BDCA-1)+ Dendritic Cell Isolation Kit has been developed for the isolation of CD1c (BDCA-1)+ myeloid dendritic cells (MDCs; type-1 MDCs or MDC1s) from PBMCs. |
性能| 供应商 | Miltenyi |
| 背景 | The CD1c (BDCA-1) antigen is specifically expressed on dendritic cells, which are CD11chighCD123low and represent the major subset of MDCs in human blood (about 0.6 % of all PBMCs).1–7 CD1c (BDCA-1)+ MDCs show a monocytoid morphology and express myeloid markers such as CD13 and CD33 as well as Fc receptors such as CD32, CD64, and FceRI. Furthermore, they were determined to be CD4+, Lin (CD3, CD16, CD19, CD20, CD56)–, CD2+, CD45RO+, CD141 (BDCA-3)low, CD303 (BDCA-2)–, and CD304 (BDCA-4/Neuropilin-1)–.1 A minor proportion of CD1c (BDCA-1)+ MDCs expresses CD14 and CD11b. CD1c (BDCA-1) is also found on CD1a+ dendritic cells generated ex vivo from monocytes or hematopoietic precursor cells. In blood, apart from MDCs, a subset of B cells also expresses CD1c (BDCA-1). Therefore, the CD1c (BDCA-1)+ Dendritic Cell Isolation Kit includes CD19 MicroBeads for depletion of B cells prior to the enrichment of CD1c (BDCA-1)+ MDCs. In order to discriminate the CD1c (BDCA-1)+ from CD1c (BDCA-1)– CD141 (BDCA-3)++ MDCs, the former have been designated type-1 MDCs (MDC1s). |
