| 货号 | 4097S |
| 描述 | This Cell Signaling Technology monoclonal antibody is conjugated to biotin under optimal conditions. |
| 来源宿主 | Mouse IgG1 |
| 应用 | IP/F |
| 使用方法 | F () |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. |
| 存放说明 | -20C |
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Akt (pan) (C67E7) Rabbit mAb #4691.免疫沉淀分析采用LY294002 #9901 (1) 或 Calyculin A #9902 (2),处理的Jurkat细胞提取物，所用抗体为Akt (pan) (40D4) Mouse mAb (生物素化) #4821 and Mouse MOPC-21) mAb IgG1同型对照(生物素化)。免疫复合物采用Immobilized Streptavidin（结合微球） #3419沉淀。Western blotting分析采用Akt (pan) (C67E7) Rabbit mAb #4691 。 | |
Flow cytometric analysis of Jurkat cells using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) (red).流式细胞术分析Jurkat细胞，所用抗体为Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 (蓝色) ，与Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) (红色)作比较。 | |
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) #5102 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.免疫沉淀分析采用LY294002 #9901 (1) 或 Calyculin A #9902 (2),处理的Jurkat细胞提取物，所用抗体为Phospho-Akt (Ser473) (587F11) Mouse mAb (生物素化) #5102 和 Mouse (MOPC-21) mAb IgG1 同型对照(生物素化)。免疫复合物采用Immobilized Streptavidin（结合微球） #3419被破坏。Western blotting分析采用Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060。 |
