| 货号 | 2701L |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. |
| 使用方法 | WB(1:1000) F (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70 Zap-70, 72 Syk |
| 参考文献 | 1 . Chu, D.H. et al. (1998) Immunol Rev 165, 167-80. 2 . Iwashima, M. et al. (1994) Science 263, 1136-9. 3 . Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8. 4 . Chan, A.C. et al. (1995) EMBO J 14, 2499-508. 5 . Williams, B.L. et al. (1999) EMBO J 18, 1832-44. 6 . Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94. 7 . Wiestner, A. et al. (2003) Blood 101, 4944-51. 8 . Crespo, M. et al. (2003) N Engl J Med 348, 1764-75. 9 . Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15. |
Western blot analysis of extracts from Jurkat cells, starved for 16 hours, and treated with 2 mM H2O2 or with calf intestinal alkaline phosphatase (CIP), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (upper) or control Zap-70 Antibody #2702 (lower).Western免疫印迹。用Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (上图) 或者 control Zap-70 Antibody #2702 (下图)研究经16小时饥饿并用2 mM H2O2或者牛肠内碱性磷酸酶 (CIP)处理的Jurkat细胞的细胞提取液。 | |
Two-color flow cytometric analysis of Jurkat cells, untreated (left) or anti-CD3 activated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody and Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) mAb #9106. Anti-CD3 activation increases the intensity of label with both antibodies.双色通道流式细胞仪研究未经处理(左图)或经CD3 激活(右图)的Jurkat细胞，所用抗体为Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody 和 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) mAb #9106.Anti-CD3激活能激活这两个抗体的标记效率。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or CD3 treated (green), using Phospho-Zap70 (Tyr319)/Syk (Tyr352) Antibody compared to a nonspecific negative control antibody (red).流式细胞仪研究未经处理的(蓝色)或经CD3处理的Jurkat细胞(绿色)。所用抗体为Phospho-Zap70 (Tyr319)/Syk (Tyr352) Antibody,非特异性的抗体为阴性对照。 | |
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-IgM (12 µg/ ml for 2 minutes), hydrogen peroxide (10 mM for 2 minutes) or lambda phosphatase, using Phospho-Zap-70 (Tyr319)/ Syk (Tyr352) Antibody.Western免疫印迹。用Phospho-Zap-70 (Tyr319)/ Syk (Tyr352) Antibody研究未经处理的和经anti-IgM (12 µg/ ml ,2 min), 过氧化氢 (10 mM,2 min)或 lambda 磷酸酶处理的Ramos细胞的细胞提取液。 | |
Immunofluorescent analysis of Jurkat cells, CD3-treated (left) or untreated (right), using Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).免疫荧光分析CD3处理的(左图) 或未经处理(右图)的Jurkat细胞的细胞提取液，所用抗体为Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody (红色) Blue pseudocolor = DRAQ5™ (DNA 荧光染料)。 |
