| 货号 | 5445S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/F |
| 使用方法 | WB(1:1000) IP (1:200) F (1:1600) |
| 供应商 | CST |
| 背景 | SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
Western blot analysis of extracts from K-562 cells, untreated or treated with λ phosphatase, using Phospho-SHIP2 (Tyr1135) (D33C6) XP® Rabbit mAb (upper) or total SHIP2 (C76A7) Rabbit mAb #2839 (lower).Western blot方法检测细胞提取物：未经处理和λ磷酸酶处理的K-562细胞。使用的抗体是Phospho-SHIP2 (Tyr1135) (D33C6) XP® Rabbit mAb (上图)和total SHIP2 (C76A7) Rabbit mAb #2839 (下图)。 | |
Flow cytometric analysis of K-562 cells, untreated (green) or λ phosphatase-treated (blue), using Phospho-SHIP2 (Tyr1135) (D33C6) XP® Rabbit mAb, compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).Flow cytometric方法检测细胞提取物：未处理的（绿色）和λ磷酸酶处理的（蓝色）K-562细胞，使用的抗体是Phospho-SHIP2 (Tyr1135) (D33C6) XP® Rabbit mAb，红色曲线表示使用的抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900。 |
