| 货号 | 4041S |
| 描述 | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody exhibits the same species cross-reactivity as the unconjugated CD44 (156-3C11) Mouse mAb #3570. |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/F |
| 目标/特异性 | CD44 (156-3C11) Mouse mAb (Biotinylated) detects endogenous levels of total CD44 protein. |
| 使用方法 | WB(1:1000) F (1:400) |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from HeLa and PANC-1 cells using CD44 (156-3C11) Mouse mAb (Biotinylated) and developed using Streptavidin-HRP #3999.Western免疫印迹。用CD44 (156-3C11) Mouse mAb (Biotinylated) 研究HeLa和PANC-1细胞的细胞提取液，并用链霉亲和素-HRP #3999显色。 | |
Flow cytometric analysis of HeLa cells using CD44 (156-3C11) Mouse mAb (Biotinylated) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097 (red).流式细胞仪分析Hela细胞，所用抗体为CD44 (156-3C11) Mouse mAb (Biotinylated)(蓝色) 与Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097 对比 (红色)。 |
