| 货号 | 9361S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-Stat6 (Tyr641) Antibody detects endogenous levels of Stat6 only when phosphorylated at tyrosine 641. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins. |
| 使用方法 | WB(1:1000) IP (1:100) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of extracts from Daudi cells, treated with IL-4 (100 ng/ml) for the indicated times using Phospho-Stat6 (Tyr641) Antibody (upper) or Stat6 antibody (lower).Western免疫印迹。用Phospho-Stat6 (Tyr641) 抗体 (上图) 或 Stat6 抗体 (下图)研究经过 IL-4 (100 ng/ml) 处理一定时间的Daudi细胞的细胞提取液。 | |
Flow cytometric analysis of Ramos cells, untreated (blue) or IL-4 treated (green), using Phospho-Stat6 (Tyr641) Antibody compared to a nonspecific negative control antibody (red).流式细胞仪分析未经处理(蓝色)和经过 IL-4处理(绿色)的Ramos细胞。所用抗体为 Phospho-Stat6 (Tyr641) Antibody 与非特异性的阴性对照抗体(红色)。 | |
Confocal immunofluorescent analysis of ACHN cells serum-starved (left) or IL-4-treated (right) using Phospho-Stat6(Tyr641) Antibody (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光。用Phospho-Stat6(Tyr641) 抗体 (绿色)分析血清饥饿(左图)或者IL-4处理(右图)。DRAQ5® #4084 (DNA荧光染料)(蓝色)。 |
