| 货号 | 2314S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-SirT1 (Ser47) Antibody detects endogenous levels of SirT1 protein only when phosphoryated at serine 47. The antibody does not cross-react with other sirtuin proteins. |
| 使用方法 | WB(1:2000) IP (1:25) F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7) and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of 293 cell lysates treated or untreated with λ phosphatase, using Phospho-SirT1 (Ser47) Antibody #2314 (upper) or SirT1 Antibody #2310 (lower). 使用Phospho-SirT1 (Ser47) Antibody #2314 (上图)或SirT1 Antibody #2310 (下图)，免疫印迹(Western blot)分析293细胞中Phospho-SirT1 (Ser47)和SirT1的蛋白水平。 | |
Flow cytometric analysis of K-562 cells, λ phosphatase treated (blue) or untreated (green), using Phospho-SirT1 (Ser47) Antibody compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用Phospho-SirT1 (Ser47) Antibody进行流式细胞仪分析K-562细胞，细胞分为λ phosphatase treated (蓝色)或untreated (绿色)。 | |
Confocal immunofluorescent analysis of HT-1080 cells, untreated (left) and phosphatase-treated (right), using Phospho-SirT1 (Ser47) Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). 使用Phospho-SirT1 (Ser47) Antibody (绿色)标记，共聚焦免疫荧光分析HT-1080细胞，细胞分为untreated (左图)和phosphatase-treated (右图)。DY-554 phalloidin标记微丝蛋白(红色)。 |
