| 货号 | 4981S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 使用方法 | WB(1:1000) IHC-P (1:150) F (1:25) |
| 供应商 | CST |
| 背景 | The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription intiation site that yields a 40 kDa deltaNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms (3,4).p53肿瘤抑制蛋白在细胞DNA损伤和其他基因组异常的应答中起着重要的作用。 p53的激活可导致无论是细胞周期停滞和DNA修复或细胞凋亡（1）。除了p53，哺乳动物细胞中含有两个p53家族成员，p63和p73，其结构和功能都与p53蛋白类似（2）。虽然p63蛋白可诱导p53-应答基因与细胞凋亡，突变的p6 3很少引起肿瘤（2）。肺部和头颈部鳞状细胞癌中经常可以观察到p63基因的扩增（2,3）。 p63基因包含一个选择性的转录起始位点，该位点能够产生缺乏反式激活结构域的40 kDa的deltaNp63，且在羧基末端选择性剪接产生α，β和γ异构体（3,4）。 |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Western blot analysis of extracts from untreated or nocodazole-treated (50 ng/ml, 24 h) ME-180 cells using Phospho-p63 (Ser160/162) Antibody (upper) or p63alpha Antibody #4892 (lower).Western blot方法检测非处理和诺考达唑(50 ng/ml) 处理24小时的ME-180细胞，使用的抗体为Phospho-p63 (Ser160/162) Antibody(上图)和 p63alpha Antibody #4892 (下图). | |
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus, showing nuclear localization, using Phospho-p63 (Ser160/162) Antibody.免疫组织化学方法检测石蜡包埋人食道鳞状细胞癌，显示核定位，使用的抗体为Phospho-p63 (Ser160/162) Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma, using Phospho-p63 (Ser160/162) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学方法检测石蜡包埋人食道鳞状细胞癌，使用的抗体为Phospho-p63 (Ser160/162) Antibody ，左图为共孵育对照多肽，右图为共孵育抗原特异性多肽。 | |
Immunohistochemical analysis of paraffin-embedded ME-180 cells, untreated (left) or nocodozole-treated (right), showing induced nuclear staining, using Phospho-p63 (Ser160/162) Antibody.免疫组织化学方法检测石蜡包埋 ME-180 细胞，分为非处理组（左图）和诺考达唑处理组（右图），显示诱导核显色，使用的抗体为Phospho-p63 (Ser160/162) Antibody。 | |
Flow cytometric analysis of Jurkat cells using Phospho-p63 (Ser160/162) antibody versus propidium iodide (DNA content).流式细胞术检测Jurkat细胞，使用的抗体为Phospho-p63 (Ser160/162)antibody ，碘化乙碇检测DNA含量作为对照。 |
