| 货号 | 3455S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-DRP1 (Ser616) Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease (reviewed in 6). |
| 存放说明 | -20C |
| 计算分子量 | 78-82 |
Immunoprecipitation of Phospho-DRP1 (Ser616) from HeLa cell extracts, untreated or nocodazole-treated, using Phospho-DRP1 (Ser616) Antibody followed by western blot using the same antibody. Lanes 1 & 2 are 5% input.使用Phospho-DRP1 (Ser616)抗体对未处理或经过nocodazole处理的HeLa细胞中的Phospho-DRP1 (Ser616)进行免疫共沉淀实验。Phospho-DRP1 (Ser616)抗体也同样用于western blot分析。 | |
Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated for the indicated times, using Phospho-DRP1 (Ser616) Antibody.使用Phospho-DRP1 (Ser616)抗体对未处理或经过nocodazole处理一定时间的HeLa细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902, using Phospho-DRP1 (Ser616) Antibody.使用Phospho-DRP1 (Ser616)抗体对未处理或经过Calyculin A #9902处理的HeLa细胞提取物进行western blot分析。 | |
Flow cytometric analysis of Jurkat cells using Phospho-DRP1 (Ser616) Antibody versus propidium iodide (DNA content).使用Phospho-DRP1 (Ser616)抗体和碘化丙啶（DNA content）对Jurkat细胞进行流式分析。 | |
Confocal immunofluorescent analysis of NCI-H1299 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-DRP1 (Ser616) Antibody (green). Actin filaments have been labeled with DY-554 phallodin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).未处理（左）或经过λ-phosphatase处理（右）的NCI-H1299细胞，使用Phospho-DRP1 (Ser616)抗体（绿色）进行激光共聚焦实验。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® (fluorescent DNA dye). |
