| 货号 | 4327S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | Phospho-AML1 (Ser249) Antibody detects endogenous levels of AML1 protein only when phosphorylated on Ser249. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts of TPA treated HEL cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (upper), or AML1 Antibody #4334 (lower).Western免疫印迹。用Phospho-AML1 (Ser249) Antibody (上图)或AML1 Antibody #4334 (下图)研究未经处理的和经λ phosphatase处理的TPA处理过的HEL细胞的细胞提取液。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with λ phosphatase (blue), using Phospho-AML1 (Ser249 ) Antibody compared to a nonspecific negative control antibody (red). | |
Confocal immunofluorescent analysis of HeLa cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析未经处理(绿色) 或 经λ phosphatase处理的HeLa细胞，所用抗体为Phospho-AML1 (Ser249 ) Antibody (绿色),肌丝蛋白用DY-554 phalloidin标记(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA 荧光染料) |
