| 货号 | 13109S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F/ChIP |
| 使用方法 | WB(1:1000) IP (1:100) F (1:800) IF-IC (1:800) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Flow cytometric analysis of ME-180 (green) and MCF7 (blue) cells using p63-α (D2K8X) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.使用p63-α (D2K8X) XP® Rabbit mAb对ME-180（绿色）和MCF7（蓝色）细胞进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412用作二抗。 | |
Confocal immunofluorescent analysis of ME-180 (left) and MCF7 (right) cells using p63-α (D2K8X) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用p63-α (D2K8X) XP® Rabbit mAb（绿色）对ME-180 (左)和 MCF7 (右)细胞进行激光共聚焦免疫荧光分析。使用DY-554鬼笔环肽（红）标记肌动蛋白丝。蓝色假色= DRAQ5®#4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from ME-180 (+), HaCaT (+), and MCF7 (-) cells using p63-α (D2K8X) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).使用p63-α (D2K8X) XP®Rabbit mAb（上）或β-Actin (D6A8) Rabbit mAb #8457（下）对ME-180 (+), HaCaT (+), 和MCF7 (-)细胞提取物进行western blot分析。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HaCaT cells and either 5 μl of p63-α (D2K8X) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunoprecipitation of p63-α from Me180 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p63-α (D2K8X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p63-alpha (D2K8X) XP® Rabbit mAb. |
